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| 分光光度法测定丝肽液中氨基酸总含量 | |
| 引用本文： | 黄威娜,杨勇,林苗. 分光光度法测定丝肽液中氨基酸总含量[J]. 香料香精化妆品, 2011, 0(3): 35-38 |
| 作者姓名： | 黄威娜 杨勇 林苗 |
| 作者单位： | 1. 化学化工与生物工程学院,生态纺织教育部重点实验室,东华大学,上海,201620;工业品与原材料检测技术中心,上海出入境检验检疫局,上海,200135 2. 工业品与原材料检测技术中心,上海出入境检验检疫局,上海,200135 3. 化学化工与生物工程学院,生态纺织教育部重点实验室,东华大学,上海,201620 |
| 摘 要： | 采用分光光度法测定丝肽液中氨基酸总含量,确定以甘氨酸为标准品、茚三酮为显色剂的测定方法.最佳反应条件:反应pH值为5.4,缓冲液的用量为2.0 mL,显色剂的用量为1.0 mL,沸水浴加热15 min,冷却至室温后,在最大吸收波长573 nm处测定吸光度.该方法操作简便、结果准确,重现性良好,可用于化妆品产品的检验. |
| 关 键 词： | 丝肽液 茚三酮 含量测定 分光光度法 |
Determination of Amino Acid Content in Silk Peptide Solution by Spectrophotometry |
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| HUANG Weina,YANG Yong,LIN Miao. Determination of Amino Acid Content in Silk Peptide Solution by Spectrophotometry[J]. Flavour Fragrance Cosmetics, 2011, 0(3): 35-38 | |
| Authors: | HUANG Weina YANG Yong LIN Miao |
| Affiliation: | HUANG Weina1,2 YANG Yong2 LIN Miao1(1.College of Chemistry,Chemical Engineering and Biotechnology,Key Laboratory of Science & Technology of Eco-Textile of Ministry of Education,Donghua University,Shanghai 201620,China,2.Inspection Center of Industrial Product & Raw Materials,Shanghai Entry-Exit Inspection & Quarantine Bureau,Shanghai 200135,China) |
| Abstract: | The total amount of amino acid in silk peptide solution was analyzed by spectrometer.The measuring method was investigated,using glycine as the standard substance and ninhydrin as the chromogenic agent.The optimum conditions were proved as follows: buffer solution volume 2 mL with pH of 5.4,chromogenic agent volume 1 mL,heating time 15 minutes in boiling water bath,absorption wavelength at 573 nm after the sample solutions were cooled to the room temperature.It was shown that this method was a simple,accura... |
| Keywords: | silk peptide solution ninhydrin content determination spectrophotometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |