| 冬虫夏草与蛹虫草原生质体融合初探 |
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| 引用本文： | 郭成金,赵润,朱文碧. 冬虫夏草与蛹虫草原生质体融合初探[J]. 食品科学, 2010, 31(1): 165-171. DOI: 10.7506/spkx1002-6630-201001039 |
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| 作者姓名： | 郭成金 赵润 朱文碧 |
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| 作者单位： | 1.天津师范大学化学与生命科学学院，天津市细胞遗传与分子调控重点实验室 2.农业部环境保护科研监测所 3.天津农学院教务处 |
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| 基金项目： | 天津市科学技术委员会项目(06ZHCXNC05900) |
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| 摘 要： | 采用正交设计和双灭活标记方法对冬虫夏草与蛹虫草进行原生质体融合研究，获得融合子。结果表明：最适融合条件为：40% PEG 与0.08mol/L Ca2+ 的混合溶液(pH7.0)助融，35℃融合20min，融合率为2.74 × 10-5；并用形态学、颉颃实验及分子生物学方法初步探讨了双亲和融合子三者之间的亲缘关系。
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| 关 键 词： | 冬虫夏草 蛹虫草 融合子 亲缘关系 |
| 收稿时间： | 2009-03-25 |
Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris |
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| GUO Cheng-jin,ZHAO Run,ZHU Wen-bi. Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris[J]. Food Science, 2010, 31(1): 165-171. DOI: 10.7506/spkx1002-6630-201001039 |
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| Authors: | GUO Cheng-jin ZHAO Run ZHU Wen-bi |
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| Affiliation: | (1. College of Chemistry and Life Science, Tianjin Normal University, Tianjin Key Laboratory of Cyto-Genetical and MolecularRegulation, Tianjin 300387, China；2. Place of Scientific Research and Monitoring on Agricultural Environmental Protection,Tianjin 300191, China；3. Dean's Office, Tianjin Agricultural University, Tianjin 300384, China) |
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| Abstract: | Fusion between protoplasts from Cordyceps sinensis and Cordyceps militaris subjected to heat and ultraviolet inactivations, respectively, was studied. In order to maximize fusion rate, five crucial fusion conditions were optimized using orthogonal array design. A maximum fusion rate of 2.74 × 10-5 was achieved through 20 min fusion at 35 ℃ under the promotion of 40% PEG and 0.08 mmol/L CaCl2 (pH 7). Besides, the genetic relationships among the parent and fusant strains were quested by morphologic observation, rivalry experiments and RAPD-PCR identification. |
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| Keywords: | Cordyceps sinensis Cordyceps militaris fusant genetic relationship |
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