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Enhancement of hepatitis B surface antigen production by adenosine 5'-monophosphate in culture of recombinant Chinese hamster ovary cells |
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| Authors: | Luo Weiwen Sun Xiangming Yi Xiaoping Zhang Yuanxing |
| Affiliation: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
| Abstract: | Adenosine 5'-monophosphate was found effective in enhancing hepatitis B surface antigen (HBsAg) production in the culture of recombinant Chinese hamster ovary (CHO) cells, but it markedly inhibited cell growth. A culture strategy with periodical medium changes was established to increase HBsAg production and avoid the AMP-induced inhibition of cell growth. The enhanced energy supply might be one of the reasons for the increase in HBsAg production in the presence of AMP. |
| Keywords: | animal cell culture adenosine 5′-monophosphate adenosine 5′-triphosphate Chinese hamster ovary (CHO) cell hepatitis B surface antigen (HBsAg) |
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