高效液相色谱- 串联质谱法快速测定鱼肉中4 种氟喹诺酮类药物残留

建立快速测定鱼肉中4 种氟喹诺酮类药物残留的高效液相色谱- 串联质谱方法。采用酸性乙腈提取样品中的氟喹诺酮药物残留，然后用正己烷脱脂，氮吹浓缩定容后，用反相液相色谱分离，以液相色谱- 串联质谱仪测定，外标法定量。该法对4 种氟喹诺酮类药物的线性范围为2～40μg/kg，相关系数(r2)大于0.997，在4、10、20μg/kg 3 个添加水平范围内的回收率为79.9%～98.1%，相对标准偏差为2.7%～8.7%，检出限为0.1～0.4μg/kg，定量限为0.3～1.0μg/kg。结果表明，该方法简便快速、灵敏度高、重现性好、选择性强，适用于鱼肉中氟喹诺酮类药物残留的定量测定。

A Rapid High-performance Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 4 Fluoroquinolones Residues in Fish Meat

A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the simultaneous determination of 4 fluoroquinolone antibiotic residues in fish meat. In the method, analytes were extracted with acidified acetonitrile, cleaned up with n-hexane, concentrated to dryness under nitrogen, redissolved in 1 mL of mixed solution of acetonitrile and 1% formic acid (1:9, V/V), separated on a RP-HPLC column, detected by HPLC-MS/MS with positive electrospray ionization (ESI+) in ...