| 二极管阵列检测器对色谱峰纯度的分析 |
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| 引用本文： | 刘飞,刘宁,程光.二极管阵列检测器对色谱峰纯度的分析[J].分析仪器,2013(6):141-144. |
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| 作者姓名： | 刘飞 刘宁 程光 |
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| 作者单位： | [1]南京大学生命分析化学国家重点实验室,南京210093 [2]南京绿叶思科药业有限公司研发部,南京210061 |
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| 摘 要： | 二极管阵列检测器（DAD）是药物分析实验室常用的高效液相色谱检测器。该型监测器除了具备紫外可见光检测器所有的功能之外，还可以在线获取色谱峰的紫外可见光吸收谱图，并据此提供峰纯度分析功能。本文从评估峰纯度的原理入手，结合安捷伦色谱工作站和实际色谱实验，详细说明峰纯度分析的2种方法和显示形式，讨论应用峰纯度分析注意事项。
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| 关 键 词： | 二极管阵列检测器 峰纯度 |
Analysis of peak purity by diode array detector |
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| Liu Fei,Liu Ning,Cheng Guang.Analysis of peak purity by diode array detector[J].Analytical Instrumentation,2013(6):141-144. |
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| Authors: | Liu Fei Liu Ning Cheng Guang |
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| Affiliation: | 1. State key laboUniversity, Nanjing 210093, China; 2. The ,. Ltd. , Nanjing 210061,China) |
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| Abstract: | Diode array detector （DAD） is a common part of high performance liquid chromatography in the pharmaceutical analysis labs. In addition to being a fully functional ultraviolet-visible （UV-Vis） detec-tor ,DAD is also capable of recording the UV-Vis spectrum simultaneously analyzing peak purity. In this paper ,we detailedly elucidated two methods and display modes of peak purity analysis based on the evalua-tion of the principle of peak purity analysis. Besides ,the precautions of applying peak purity analysis were discussed combining with practical experiences of using Agilent HPLC workstation. |
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| Keywords: | diode array detector peak purity |
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