枸杞中维生素B2固体粉末的荧光光谱检测研究

利用日本岛津RF-5301PC荧光分光光度计,对枸杞子粉末中的维生素B2(VB2)直接进行荧光光谱检测,采集的光谱范围在530～650nm波段。采用MATLAB数值微分和高斯函数拟合方法,得到在激发波长为480nm、峰值波长为563nm时的荧光光谱信息;通过数值积分和线性拟合,得到荧光强度沿波长的积分与VB2含量呈良好的线性关系,其相关系数为0.998 43。实验测定枸杞粉末VB2含量为1.28±0.17μg/g,并阐释了枸杞子粉末荧光光谱红移的原因。研究表明:这种固体粉末荧光光谱检测方法具有快速、直接、简单和环保等优点,对药品成份检测、食品安全监测等领域的应用具有重要的参考价值。

Determination of the contents of VB2 in Lycium barbarum L.powder with fluorescence spectrum method

By using RF-5301PC series spectrofluorophotometer,the fluorescence spectrum of Lycium barbarum L.powder is directly acquired to measure the content of vitamin B2(VB2).The collected its fluorescence spectrum is in the band of 530-650 nm.Through MATLAB-based numerical differential and Gaussian fitting algorithms,the fluorescent spectrum information with 563 nm peak under 480 nm excitation has been obtained,and after numerical integral ealculation and linear fitting,it is found that there exists a linear relationship between the integral of fluorescent intensity by wavelength and the content of VB2(R=0.9998 43).The content of VB2 in Lycium barbarum L.powder is measured to be 1.28±0.17 μg/g.The cause of red shift of the fluorescence spectrum of the Lycium barbarum L.powder is illustrated.Our proposed VB2 detection method has many advantages,such as high speed,directness,simplicity,and environment protection,and it is also a powerful tool that could be applied in food security detection.