| 鄂尔多斯钝顶螺旋藻对三种抗生素敏感性研究 |
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| 引用本文： | 巩东辉,乔辰,李春霞,季祥,姜东成. 鄂尔多斯钝顶螺旋藻对三种抗生素敏感性研究[J]. 包头钢铁学院学报, 2007, 26(1): 67-69 |
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| 作者姓名： | 巩东辉 乔辰 李春霞 季祥 姜东成 |
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| 作者单位： | [1]内蒙古科技大学生物与化学工程学院,内蒙古包头014010 [2]内蒙古农业大学农学院,内蒙古呼和浩特010019 [3]包头钢铁（集团）公司第一中学,内蒙古包头014010 |
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| 摘 要： | 通过3种基因工程中常用抗生素对鄂尔多斯钝顶螺旋藻(S.erdosensis)生长抑制的研究发现,鄂尔多斯钝顶螺旋藻对氯霉素较敏感,液体培养1 mg/L的氯霉素即可抑制其生长,500 mg/L的氯霉素处理9 d产生完全致死作用;鄂尔多斯钝顶螺旋藻对氨卞青霉素和卡那霉素敏感性较弱,300 mg/L的氨卞青霉素和卡那霉素对鄂尔多斯钝顶螺旋藻生长的抑制不明显.结果表明,氯霉素是转化系统中较理想的抗性选择剂.
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| 关 键 词： | 螺旋藻 抗生索 敏感 鄂尔多斯 钝顶螺旋藻 常用抗生素 敏感性研究 antibiotics Spirulina sensitivity 选择剂 抗性 转化系统 结果 卡那霉素 青霉素 致死作用 完全 处理 生长抑制 液体培养 氯霉素 发现 |
| 文章编号： | 1004-9762（2007）001-0067-03 |
| 收稿时间： | 2006-11-17 |
| 修稿时间： | 2006-11-17 |
Study on the sensitivity of Spirulina erdosensis to three antibiotics |
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| GONG Dong-hui,QIAO Chen,LI Chun-xia,JI Xiang,JIANG Dong-cheng. Study on the sensitivity of Spirulina erdosensis to three antibiotics[J]. Journal of Baotou University of Iron and Steel Technology, 2007, 26(1): 67-69 |
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| Authors: | GONG Dong-hui QIAO Chen LI Chun-xia JI Xiang JIANG Dong-cheng |
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| Abstract: | Three different antibiotics were tested to determine Spirulina erdosensis special sensitivity as a foundation of selecting the resistant gene and constituting expression system of Spirulina erdosensis for recombinant foreign gene. The results revealed that Spirulina erdosensis was highly sensitive to Cm, and 1 mg/L Cm could inhibit the growth of Spirulina erdosensis,while 500 mg/L Cm could kill the algae in 9 d; Spirulina erdosensis was not sensitive to Amp and Kin,as 300 mg/L Amp and Km could not significantly restrain Spirulina erdosensis from growing.It is suggested that Cm is a better selective marker for the gene transfer of Spirulina erdosensis. |
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| Keywords: | spirulina antibiotics sensitivity |
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