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| 新型纳米基因载体(PEI/Mn0.5Zn0.5Fe2O4)的制备、表征及体外实验 | |
| 引用本文： | 唐秋莎,张东生,顾宁,丛小明,万美玲,金立强.新型纳米基因载体(PEI/Mn0.5Zn0.5Fe2O4)的制备、表征及体外实验[J].功能材料,2007,38(8):1268-1272. |
| 作者姓名： | 唐秋莎 张东生 顾宁 丛小明 万美玲 金立强 |
| 作者单位： | 1. 东南大学,临床医学院,江苏,南京,210009 2. 东南大学,临床医学院,江苏,南京,210009;东南大学,基础医学院,江苏省生物材料和器件重点实验室,江苏,南京,210009 3. 东南大学,生物科学与医学工程学院,江苏省生物材料和器件重点实验室,江苏,南京,210096 4. 东南大学,基础医学院,江苏省生物材料和器件重点实验室,江苏,南京,210009 |
| 基金项目： | 国家自然科学基金 , 国家高技术研究发展计划(863计划) |
| 摘 要： | 采用改良的化学共沉淀法制备锰锌铁氧体磁性纳米粒子,聚乙烯亚胺(PEI)对其进行表面修饰.利用XRD、EDS、TEM、SEM、FTIR、XPS、UV-vis、Zeta电位仪、电泳仪、荧光显微镜等手段对其形貌、物象、成份、表面包覆功能团、元素组成、表面电位、磁响应性、DNA结合保护、体外释放及转染能力进行表征.体外加热实验验证其升温恒温能力.XRD及EDS确认已成功制备锰锌铁氧体磁性纳米粒子.修饰后的磁性纳米粒子具有良好的分散性、磁响应性及升温恒温能力.红外光谱及XPS分析验证了PEI在磁性纳米粒子表面的吸附.修饰后磁性纳米粒子的等电点由pH=7.0移至pH=11.0.具有良好的DNA结合保护转染能力.有利于其在生物医学领域的应用. |
| 关 键 词： | 磁性纳米粒子 锰锌铁氧体 基因载体 |
| 文章编号： | 1001-9731（2007）08-1268-05 |
| 修稿时间： | 2007-01-042007-04-27 |
Synthesis and in vitro study of PEI-coated Mn-Zn ferrite——A novel gene vector |
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| TANG Qiu-sha,ZHANG Dong-sheng,GU Ning,CONG Xiao-ming,WAN Mei-lin,JIN Li-qiang.Synthesis and in vitro study of PEI-coated Mn-Zn ferrite——A novel gene vector[J].Journal of Functional Materials,2007,38(8):1268-1272. | |
| Authors: | TANG Qiu-sha ZHANG Dong-sheng GU Ning CONG Xiao-ming WAN Mei-lin JIN Li-qiang |
| Affiliation: | 1.School of Clinical Medical Science,Southeast University,Nanjing 210009,China;2.School of Biological Science and Medical Engineering,Jiangsu Key Lab.of Biomaterials Southeast University,Nanjing 210096,China;3.School of Basic Medical Science,Southeast University,Jiangsu Key Lab.of Biomaterials,Nanjing 210009,China |
| Abstract: | Mn-Zn ferrite Magnetic nanoparticles(MZF-NPs) were prepared through co-precipitation method.The surface of particles was modified by PEI.The appearance,structure and phase constitute,function groups and charge of the surface;elemental analysis,magnetic susceptibility of MNPs;release progress in vitro;loading capability,protection ability and transfect capability to DNA were characterized by XRD,EDS,TEM,SEM,FTIR,XPS,Zeta potential and visible light spectrophotometer etc.Thermodynamic test of various doses of nanoparticles were done.After modified,the MNPs present favorable dispensability,responsibility to magnetism,good loading capability,transfect capability and protection ability to DNA.In AMF,they can rise to a steady temperature.IR spectra and XPS spectra were obtained to verify the PEI adsorption effect.Beside,the IEP of PEI-coated MNPs changed from pH=7.0 to pH=11.0. |
| Keywords: | magnetic nanoparticles(MNPs) Mn-Zn ferrite gene vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |