| 碳氮源对酶法合成L-半胱氨酸的影响 |
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| 引用本文： | 怀丽华,Kou Guang-hui,寇广会,周昌平,陈宁. 碳氮源对酶法合成L-半胱氨酸的影响[J]. 现代食品科技, 2006, 22(4): 85-87 |
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| 作者姓名： | 怀丽华 Kou Guang-hui 寇广会 周昌平 陈宁 |
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| 作者单位： | 天津商学院,天津,300134;天津科技大学生物工程学院,天津,300222;天津科技大学生物工程学院,天津,300222 |
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| 基金项目： | 天津市科委应用基础研究重点基金 |
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| 摘 要： | 以假单胞菌(Pseudomonas sp.)TS1138为供试菌株,研究了在酶法转化DL-ATC合成L-半胱氨酸中碳氮源对酶源细胞酶活力的影响,确定了在产酶培养基中最适的碳源是葡萄糖,最适加入量为4.0%;最适的氮源是酵母粉、蛋白胨和尿素的组合,最适加入量分别是蛋白胨1.0%,酵母粉1.5%,尿素0.3%,酶源细胞的酶活力达到2679 U·mL-1.
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| 关 键 词： | L-半胱氨酸 酶法合成 酶活力 |
| 文章编号： | 1007-2764(2006)04-0085-026 |
| 修稿时间： | 2006-06-16 |
Effects of Carbon and Nitrogen Source on Enzymatic Synthesis of L-cysteine |
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| Kou Guang-hui. Effects of Carbon and Nitrogen Source on Enzymatic Synthesis of L-cysteine[J]. Modern Food Science & Technology, 2006, 22(4): 85-87 |
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| Authors: | Kou Guang-hui |
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| Abstract: | Pseudomonas sp. TS1138 was used as the test strain to produce L-cysteine,and the effect of carbon and nitrogen source on enzyme activity was studied. In the medium optimization,it was found that glucose was the best carbon source,and the optimum concentration was 4.0%,and nitrogen source were peptone,yeast extract and urea,the optimum concentration were 1.0%,1.5%,0.3% respectively. Enzyme activity could reach 2679 U?mL-1 under the optimum condition. |
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| Keywords: | L-cysteine Enzymatic synthesis Enzyme activity |
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