| 小球藻糖蛋白的分离纯化与性质测定 |
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| 引用本文： | 魏文志,夏文水,李湘鸣,吴玉娟. 小球藻糖蛋白的分离纯化与性质测定[J]. 食品科学, 2006, 27(11): 101-104 |
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| 作者姓名： | 魏文志 夏文水 李湘鸣 吴玉娟 |
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| 作者单位： | 江南大学食品学院； 扬州大学医学院； 扬州大学动物科学与技术学院 |
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| 摘 要： | 小球藻经热水抽提、脱蛋白、乙醇沉淀得粗糖蛋白,粗糖蛋白经Sephadex-75层析得到糖蛋白纯品。糖蛋白纯品经Sephadex-200层析证明为单一物质。紫外可见光谱只出现280nm和195nm的单峰。SDS-聚丙烯酰胺凝胶电泳显示糖蛋白分子量为63.7kDa。气相色谱分析表明该糖蛋白单糖组成主要是葡萄糖、半乳糖、鼠李糖。β-消去反应证明糖和蛋白是O-糖肽键连接的。
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| 关 键 词： | 小球藻 糖蛋白 分离纯化 性质 |
| 文章编号： | 1002-6630（2006）11-0101-04 |
| 收稿时间： | 2006-08-30 |
| 修稿时间： | 2006-08-30 |
Isolation, Purification and Characterization of the Glycoprotein from Chlorella Pyrenoidosa |
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| WEI Wen-zhi,XIA Wen-shui,LI Xiang-ming,WU Yu-juan. Isolation, Purification and Characterization of the Glycoprotein from Chlorella Pyrenoidosa[J]. Food Science, 2006, 27(11): 101-104 |
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| Authors: | WEI Wen-zhi XIA Wen-shui LI Xiang-ming WU Yu-juan |
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| Affiliation: | 1.School of Food Science and Technology,Southern Yangtze University, Wuxi 214036, China; 2.College of Animal Science and Technology,Yangzhou University, Yangzhou 225009, China; 3 Medical College, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | The crude glycoprotein was extracted from Chlorella Pyrenoidosa with hot water, and precipitated by ethanol after deproteinization. A refined glycoproteins were prepared after further purification on Sephadex-75 column chromatography, and their homogeneity was examined with Sephadex-200 column chromatography. The ultraviolet spectra showed their characteris- tic absorption peak at 280.00nm and 195.00nm. The molecular weight was estimated to be about 63.7kDa on SDS-PAGE. It was proved that the main monosacchrides were glucose, galactose and xylose by gas chromatography, and O-glycosidic linkage was found by β-elimination reaction. |
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| Keywords: | ChloreUa Pyrenoidosa glycoprotein isolation and purification characterization |
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