| 连续流动分析法测定烟草中的蛋白质 |
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| 引用本文： | 瞿先中,程涛,蒋士盛,鲍穗. 连续流动分析法测定烟草中的蛋白质[J]. 烟草科技, 2006, 0(1): 41-42 |
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| 作者姓名： | 瞿先中 程涛 蒋士盛 鲍穗 |
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| 作者单位： | 芜湖卷烟厂技术中心,安徽省芜湖市芜石路135号,241002 |
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| 摘 要： | 先用0.5%醋酸煮沸样品15min,抽滤,滤渣用0.5%醋酸洗涤,再将滤渣置于消化管中,加入0.1g HgO1、.0g K2SO4和5mL浓硫酸消化,然后采用连续流动分析法测定烟叶样品中的蛋白质含量。方法的RSD为1.1%,回收率在98%~102%之间。并采用该法测定了部分烟叶的蛋白质含量。
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| 关 键 词： | 烟草 连续流动分析法 蛋白质 |
| 文章编号： | 1002-0861(2006)01-0041-03 |
| 修稿时间： | 2005-11-28 |
Determination of Protein in Tobacco with Autoanalyzer |
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| QU XIAN-ZHONG,CHENG TAO,JIANG SHI-SHENG,BAO SUI. Determination of Protein in Tobacco with Autoanalyzer[J]. Tobacco Science & Technology, 2006, 0(1): 41-42 |
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| Authors: | QU XIAN-ZHONG CHENG TAO JIANG SHI-SHENG BAO SUI |
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| Abstract: | The tobacco sample was extracted with 0.5% acetic acid boiled for fifteen minutes,filtrated,and washed with 0.5% acetic acid,during which the water soluble nitrogen was removed.Then,the sample residue was(digested) in the presence of 0.1g HgO,1.0g K_2SO_4 and 5mL concentrated sulphuric acid.The protein in resultant digestion solution was determined with an autoanalyzer.The results showed that the relative standard deviation of the method was 1.1%,and the recoveries were from 98% to 102%.The protein in some tobacco leaves were also(determined). |
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| Keywords: | Tobacco Autoanalyzer Protein |
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