Hydrolysis degree,peptide profile and phenylalanine removal from whey protein concentrate hydrolysates obtained by various proteases |
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Authors: | Marialice P. C. Silvestre Maitê C. da Silva Mariana W. S. de Souza Viviane D. M. Silva Marcos J. B. de Aguiar Mauro R. Silva |
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Affiliation: | 1. Universidade Federal de Minas Gerais (UFMG), , Belo Horizonte, Minas Gerais, Brazil;2. EDETEC Indústria Alimentícia S/A, , Belo Horizonte, Minas Gerais, Brazil |
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Abstract: | The action of various proteases was tested for preparing whey protein concentrate (WPC) hydrolysates with high degree of hydrolysis (DH), appropriate peptide profiles and reduced phenylalanine (Phe) content. The peptide profile analysis included the fractionation of hydrolysates by size‐exclusion HPLC. The rapid correct fraction area method was used to quantify the components of the chromatographic fractions. Activated carbon (AC) was used to remove Phe, and its efficiency was evaluated by measuring the amount of Phe by second‐derivative spectrophotometry. The results showed that the DH of WPC hydrolysates increased and that the protease from Aspergillus oryzae yielded the highest DH value. This protease also produced the best peptide profile, that is, the highest di‐ and tripeptide content (16.14%), the highest amounts of free amino acids (18.43%) and the lowest amount of large peptides (18.76%). The proteases from both A. oryzae and Bacillus subtilis produced the highest Phe removals (79.0 and 77.8%, respectively). |
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Keywords: | Activated carbon enzymatic treatment phenylalanine removal protein hydrolysate size‐exclusion HPLC whey protein concentrate |
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