Human NKCC2 cation–Cl– co‐transporter complements lack of Vhc1 transporter in yeast vacuolar membranes |
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Authors: | Silvia Petrezselyova Angel Dominguez Pavla Herynkova Juan F Macias Hana Sychrova |
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Affiliation: | 1. Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, , Prague, Czech Republic;2. Department of Microbiology and Genetics, IBSAL, CIETUS, University of Salamanca, , Spain;3. Department of Medicine, University of Salamanca, , Spain |
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Abstract: | Cation–chloride co‐transporters serve to transport Cl– and alkali metal cations. Whereas a large family of these exists in higher eukaryotes, yeasts only possess one cation–chloride co‐transporter, Vhc1, localized to the vacuolar membrane. In this study, the human cation–chloride co‐transporter NKCC2 complemented the phenotype of VHC1 deletion in Saccharomyces cerevisiae and its activity controlled the growth of salt‐sensitive yeast cells in the presence of high KCl, NaCl and LiCl. A S. cerevisiae mutant lacking plasma‐membrane alkali–metal cation exporters Nha1 and Ena1‐5 and the vacuolar cation–chloride co‐transporter Vhc1 is highly sensitive to increased concentrations of alkali–metal cations, and it proved to be a suitable model for characterizing the substrate specificity and transport activity of human wild‐type and mutated cation–chloride co‐transporters. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | heterologous expression CCC family alkali– metal cation tolerance Saccharomyces cerevisiae ion homeostasis |
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