Vitamin E reduces cholesterol esterification and uptake of acetylated low density lipoprotein in macrophages |
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Authors: | Hideki Shige Toshitsugu Ishikawa Michio Suzukawa Masato Nishiwaki Takeshi Yamashita Kei Nakajima Toshimitsu Ito Kenji Higashi Makoto Ayaori Atsushi Yonemura Paul Nestel Haruo Nakamura |
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Affiliation: | (1) First Department of Internal Medicine, National Defense Medical College, Commercial Road, 359 Tokorozawa Saitama, Japan;(2) Cardiovascular Nutrition Laboratory, Baker Medical Research Institute, 3181 Prahran, Victoria, Australia |
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Abstract: | The effects of vitamin E on cholesteryl ester (CE) metabolism in 1774 cells were examined. Pretreatment of 1774 cells with
vitamin E at concentrations above 50 μM significantly decreased acetylated low density lipoprotein (LDL)-induced incorporation
of 14C]oleate into CF in cells in a dose-dependent manner. This was partly due to vitamin E Also significantly inhibiting the uptake
of 3H]CE-labeled acetylated LDL by 1774 cells. A trend existed toward suppression of acyl-CoA:cholesterol acyltransferase (ACAT)
activity in the cell lysate at high vitamin E concentration, but there was no effect on hydrolysis of CE. These data indicate
that vitamin E reduces the uptake of modified LDL and suppresses ACAT activity, resulting in less cholesterol esterification
in macrophages; a novel mechanism underlying the antiatherogenic properties of vitamin E. |
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