二氯二茂钛二甲硫氨酸与DNA相互作用的电化学及紫外-可见光谱研究

用循环伏安法考察了二茂钛二甲硫氨酸配合物(TMC)与DNA在pH=3.0的KCl水溶液中相互作用的电化学行为。TMC在玻碳电极上分别于-412 mV和-528 mV(υs.SCE)有一个氧化峰和相应的还原峰。加入DNA后,TMC的式量电位负移5 mV,峰电流增加,电化学参数(α和ks)改变。结果表明:在该条件下,TMC与DNA之间通过静电作用,形成了一种具有电活性的复合物,且TMC与DNA之间的结合位点数n和结合常数K分别为4.36和2.74×1016。结合紫外-可见光谱法测定结果,加入DNA后TMC的紫外-可见光谱在393 nm处呈现增色效应,进一步确证了TMC与DNA之间主要以静电作用为主。

Studies on the Interaction between Titanocene Di(L-Methionine) Dichloride and DNA by Cyclic Voltametry and UV-Vis Spectrometry

The electrochemical behavior of titanocene di(L-methionine) dichloride(TMC) and deoxyribonucleic acid(DNA) at pH 3.0 was investigated by cyclic Voltametry(CV).TMC yielded an anodic peak at-412 mV and a cathodic peak at-528 mV on the glassy carbon electrode.After the addition of DNA,the formal potential of TMC negatively shifted 5 mV,the peak current of TMC increased and the electrochemical parameters(such as charge transfer coefficient α and standard electron transfer rate constant k s) changed.The CV results showed that the electroactive complex was formed via electrostatic effects at pH 3.0.Moreover,the binding number n of 4.36 and an affinity constant K of 2.74×1016 were determined.Hyperchromism was observed in the UV-Vis spectra of TMC in the presence of the DNA.The results obtained from UV-Vis spectrometry further confirmed that the complex of TMC and DNA was formed by electrostatic effects.