| 马铃薯天冬氨酸蛋白酶抑制剂与DNA的相互作用及抗氧化作用 |
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| 引用本文： | 孙莹,田亚平.马铃薯天冬氨酸蛋白酶抑制剂与DNA的相互作用及抗氧化作用[J].食品与生物技术学报,2011,30(3):435-439. |
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| 作者姓名： | 孙莹 田亚平 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏无锡,214122 |
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| 基金项目： | 国家"十一五"科技支撑计划项目 |
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| 摘 要： | 从马铃薯中纯化一种天冬氨酸蛋白酶抑制剂(PPI)并对其与DNA的相互作用及抗氧化作用进行研究。以溴化乙啶作为探针,利用荧光法在初步筛选抗癌药物上的应用,首次对PPI与DNA的相互作用进行了研究;利用二苯代苦味酰基自由基体系、H2O2/Fe2+产生的羟基自由基体系、超氧阴离子自由基体系对PPI的体外清除自由基的能力进行研究,结果表明PPI具有一定体外抗氧化活性且从与DNA作用的角度分析可能具有抗癌效果。
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| 关 键 词： | 马铃薯天冬氨酸蛋白酶抑制剂 荧光谱 抗癌活性 抗氧化活性 |
Study on the Interaction with DNA and Antioxidant Activity of the Aspartic Protease Inhibitors from Potato |
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| SUN Ying and TIAN Ya-ping.Study on the Interaction with DNA and Antioxidant Activity of the Aspartic Protease Inhibitors from Potato[J].Journal of Food Science and Biotechnology,2011,30(3):435-439. |
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| Authors: | SUN Ying and TIAN Ya-ping |
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| Affiliation: | SUN Ying,TIAN Ya-ping(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | The purpose of this study was to purify a kind of aspartic protease inhibitor from potato and investigate its interaction with DNA and antioxidant activity.With ethidium bromide as a probe,Fluorescence method for screening anticancer drugs in the application,the first time the interaction between PPI and DNA has been studied;Use scavenging of free radicals of flavonoids,DPPH,H2O2/Fe2+ for hydroxyl radical system and superoxide anion radical system of the PPI in vitro antioxidant activity study,The results s... |
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| Keywords: | Aspartic protease inhibitor fluorescence spectrum anticancer activity antioxidant activity |
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