| L-缬氨酸高产菌诱变育种的研究 |
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| 引用本文： | 张伟国,钱和.L-缬氨酸高产菌诱变育种的研究[J].食品与生物技术学报,2011,30(3):394-397. |
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| 作者姓名： | 张伟国 钱和 |
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| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,江苏无锡,214122;江南大学生物工程学院,江苏无锡,214122
2. 江南大学食品学院,江苏无锡,214122 |
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| 摘 要： | 以黄色短杆菌(Brevibacterium flavum)L-缬氨酸产生菌XQ-2为出发菌株,经硫酸二乙酯(DES)和亚硝基胍(NTG)逐级诱变处理,氨基酸结构类似物α-氨基丁酸(-αAB)、2-噻唑丙氨酸(2-TA)和α-氨基-β-羟基戊酸(AHV)定向筛选,获得一株L-缬氨酸高产菌XQ-8(Leul(-ABhr2-TAhrAHVhr)。在以135 g/L葡萄糖为碳源和50 g/L硫酸铵为氮源的培养基中摇瓶发酵72 h,产酸达64~66 g/L。
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| 关 键 词： | L-缬氨酸 诱变育种 黄色短杆菌 |
Studies on the Breeding of L-Valine Hyper-Producing Mutant |
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| ZHANG Wei-guo and QIAN He.Studies on the Breeding of L-Valine Hyper-Producing Mutant[J].Journal of Food Science and Biotechnology,2011,30(3):394-397. |
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| Authors: | ZHANG Wei-guo and QIAN He |
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| Affiliation: | ZHANG Wei-guo1,2,QIAN He3(1.Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China,2.School of Biotechnology,3.School of Food Science and Technology,China) |
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| Abstract: | Using Brevibacterium flavum XQ-2(Leul(-ABrAHVr2-TAr) as a starting strain for stepwise mutagenesis by diethylsulfate(DES),N-methyl-N’-nitro-soguanidine(NTG) treatment,a L-valine hyper-producing mutant XQ-8(Leul(-AB hr2-TAhr AHVhr) was obtained,which could be resistant to high concentration α-amino-butyric acid(α-AB),2-thiazole-DL-alanaine(2-TA) and α-amino-β-hydroxy-valeric acid(AHV).As a result,high L-valine concentration(64~66g/L) was obtained with shaking at 31±1℃ for 72h in the medium containing 135g/L glucose and 50g/L(NH4)2SO4. |
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| Keywords: | L-valine breeding brevibacterium flavum |
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