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水提醇沉法从葵花籽中提取绿原酸
引用本文:郑喜群,蒋继丰,刘晓兰,孙秀云. 水提醇沉法从葵花籽中提取绿原酸[J]. 食品科学, 2006, 27(1): 159-161
作者姓名:郑喜群  蒋继丰  刘晓兰  孙秀云
作者单位:齐齐哈尔大学生命科学与工程学院; 中国农业大学食品科学与营养工程学院
基金项目:黑龙江省齐齐哈尔市科技局资助项目;黑龙江省高校骨干教师创新能力资助计划
摘    要:绿原酸具有抗菌、抗病毒、抗氧化等生物活性。葵花籽富含绿原酸。以水酶法提取葵花籽油脂后分离出的水浸提液为原料,采用醇沉淀法去除浸提液中的多糖,抽滤浓缩滤液后得到绿原酸。多次实验结果表明,浸提液浓缩到固形物含量在13%时,加入浸提浓缩液体积1倍量的95%的乙醇沉淀并过滤掉多糖,反复处理两次,滤液再经真空浓缩得到纯度76%左右的绿原酸。

关 键 词:葵花籽   浸提液   绿原酸   多糖  
文章编号:1002-6630(2006)01-0159-03
收稿时间:2004-10-20
修稿时间:2004-10-20

Extraction of Chlorogenic Acid from Sunflower Seeds by Water Solution and Alcohol Sedimentation
ZHENG Xi-qun,JIANG Ji-feng,LIU Xiao-lan,SUN Xiu-yun. Extraction of Chlorogenic Acid from Sunflower Seeds by Water Solution and Alcohol Sedimentation[J]. Food Science, 2006, 27(1): 159-161
Authors:ZHENG Xi-qun  JIANG Ji-feng  LIU Xiao-lan  SUN Xiu-yun
Affiliation:1.College of Life Science and Engineering, Qiqihar University; 2.College of Food Science and Nutritional Engineering, China Agricultural University.
Abstract:Chlorogenic acid has biological activity such as anti-microbes, anti-virus , oxidation prevention and so on. Sunflower seeds are full of chlorogenic acid. This experience extracted chlorogenic acid out of waste aqueous solution from water-enzyme method sunflower seeds oil extraction. The experiments results showed that when concentration of solid substance of the waste aqueous solution was condensated to 13% (W/V)on vacuum, 95% alcohol(1:1,V/V) was added to the condensated solution to sedimentate polysaccharose, sedimentation was centrifugalized, repeated above treatment, and filtrate was collected and condensated on vacuum, then chlorogenic acid crystal of 76% purity was obtained.
Keywords:sunflower seeds   aqueous solution   chlorogenic acid   polysaccharose
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