Protein topology of presenilin 1 |
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Authors: | A Doan G Thinakaran DR Borchelt HH Slunt T Ratovitsky M Podlisny DJ Selkoe M Seeger SE Gandy DL Price SS Sisodia |
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Affiliation: | Department of the History of Health Sciences, University of California, San Francisco, USA. |
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Abstract: | Mutations in a gene encoding a multitransmembrane protein, termed presenilin 1 (PS1), are causative in the majority of early-onset cases of AD. To determine the topology of PS1, we utilized two strategies: first, we tested whether putative transmembranes are sufficient to export a protease-sensitive substrate across a lipid bilayer; and second, we examined the binding of antibodies to specific PS1 epitopes in cultured cells selectively permeabilized with the pore-forming toxin, streptolysin-O. We document that the "loop," N-terminal, and C-terminal domains of PS1 are oriented toward the cytoplasm. |
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