动物源食品中β受体阻断剂残留量测定的不确定度评定

建立了液相色谱-串联质谱(LC-MS/MS)法测定动物源食品中β受体阻断剂残留量的数学模型,依据影响测量结果的不确定度来源,如标准溶液配制、样品称量、回归方程、方法重复性等,对各个不确定度分量进行评定和分析,给出了0.5μg/kg检测水平下,液相色谱-串联质谱法测定食品中β受体阻断剂残留量的相对合成标准不确定度以及扩展不确定度。通过比较各分量不确定度,发现实验过程中最小二乘法拟合带来的不确定度影响最大。

Uncertainty Evaluation in the Determination of β-Blockers in Food of Animal Origin

β-Blockers in food of animal origin was determined by liquid chromatography-tandem mass spectrometry（LC-MS/MS）. According to the mathematic model, the uncertainty sources of test results were analyzed, such as the standard solution preparation, sample weighing, regression equation, method repeatability. Each component uncertainty was calculated through analyzing. With the limits of quantification of 0.5 μg/kg for all analytes, the expanded uncertainty was calculated while the combined standard uncertainty of β-blockers residue was obtained. Comparing of each component of uncertainty in the process, the results show that the effect of the least squares fitting is the maximun.