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Identification of a microscopically selected microorganism in milk samples |
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| Authors: | Nathalie Bracke Mario Van Poucke Bram Baert Evelien Wynendaele Lobke De Bels Wim Van Den Broeck Luc Peelman Christian Burvenich Bart De Spiegeleer |
| Affiliation: | * Drug Quality and Registration (DruQuaR) Group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium;† Department of Animal Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium;‡ Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium;§ Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium |
| Abstract: | Identification of unwanted microbial contaminants microscopically observed in food products is challenging due to their low abundance in a complex matrix, quite often containing other microorganisms. Therefore, a selective identification method was developed using laser capture microdissection in combination with direct-captured cell PCR. This procedure was validated with Geobacillus stearothermophilus and further used to identify microbial contaminants present in some industrial milk samples. The microscopically observed contaminants were identified as mainly Methylobacterium species. |
| Keywords: | laser capture microdissection cultivation-independent identification bacterial contamination direct-captured cell PCR |
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