Antimicrobial activity of acid-hydrolyzed Citrus unshiu peel extract in milk |
| |
Authors: | Keun Young Min Hyun Jung Kim Kyoung Ah Lee Kee-Tae Kim Hyun-Dong Paik |
| |
Affiliation: | * Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea;† Department of Food Bioengineering, Jeju National University, Jeju 690-756, Korea;‡ Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea |
| |
Abstract: | Citrus fruit (Citrus unshiu) peels were extracted with hot water and then acid-hydrolyzed using hydrochloric acid. Antimicrobial activities of acid-hydrolyzed Citrus unshiu peel extract were evaluated against pathogenic bacteria, including Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes. Antilisterial effect was also determined by adding extracts at 1, 2, and 4% to whole, low-fat, and skim milk. The cell numbers of B. cereus, Staph. aureus, and L. monocytogenes cultures treated with acid-hydrolyzed extract for 12 h at 35°C were reduced from about 8 log cfu/mL to <1 log cfu/mL. Bacillus cereus was more sensitive to acid-hydrolyzed Citrus unshiu peel extract than were the other bacteria. The addition of 4% acid-hydrolyzed Citrus unshiu extracts to all types of milk inhibited the growth of L. monocytogenes within 1 d of storage at 4°C. The results indicated that Citrus unshiu peel extracts, after acid hydrolysis, effectively inhibited the growth of pathogenic bacteria. These findings indicate that acid hydrolysis of Citrus unshiu peel facilitates its use as a natural antimicrobial agent for food products. |
| |
Keywords: | antimicrobial effect Citrus unshiu peel acid hydrolysis flavonoid pathogen |
本文献已被 ScienceDirect 等数据库收录! |
|