用于光遗传技术的体外细胞光刺激系统

光遗传技术具有在毫秒水平上精细调控某种特定细胞亚群活性的优势,对神经回路和某些神经系统疾病的发病机制和干预治疗等基础研究提供了一个崭新的生物工程学工具。文章基于光遗传技术的特点,研发了一套针对光感基因改造过体外培养细胞与离体脑片LED光刺激系统,实现远程无线控制LED整列的刺激时间、频率、占空比等参数,并同步检测培养细胞的理化特性。

In Vitro Cell Culture Light Stimulation System for Optogenetics

Optogenetics technology has the advantage of regulating the activity of specific cell population in milliseconds level, which provides a novel bioengineering tools for understanding the mechanism of the neural circuits and the pathogenesis of some neurological diseases and interventions treatment. Based on the characteristics of optogenetics, we developed a set of LED light stimulation system for optogenetics modified in vitro cell culture or brain slice. This device can remotely control stimulation parameters of LED arrays such as time, frequency, duty cycle, and simultaneously detect physicochemical properties of cultured cells.