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Enhanced and Differential Capture of Circulating Tumor Cells from Lung Cancer Patients by Microfluidic Assays Using Aptamer Cocktail |
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| Authors: | Libo Zhao Chuanhao Tang Li Xu Zhen Zhang Xiaoyan Li Haixu Hu Si Cheng Wei Zhou Mengfei Huang Anna Fong Bing Liu Hsian‐Rong Tseng Hongjun Gao Yi Liu Xiaohong Fang |
| Affiliation: | 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Zhongguancun, Beijing, P. R. China;2. Department of Lung Cancer, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, P. R. China;3. Laboratory of Oncology, Translational Medicine Center, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, P. R. China;4. Department of Molecular and Medical Pharmacology, California NanoSystems Institute, University of California, Los Angeles, CA, USA |
| Abstract: | Collecting circulating tumor cells (CTCs) shed from solid tumor through a minimally invasive approach provides an opportunity to solve a long‐standing oncology problem, the real‐time monitoring of tumor state and analysis of tumor heterogeneity. However, efficient capture and detection of CTCs with diverse phenotypes is still challenging. In this work, a microfluidic assay is developed using the rationally‐designed aptamer cocktails with synergistic effect. Enhanced and differential capture of CTCs for nonsmall cell lung cancer (NSCLC) patients is achieved. It is also demonstrated that the overall consideration of CTC counts obtained by multiple aptamer combinations can provide more comprehensive information in treatment monitoring. |
| Keywords: | aptamers cancer diagnosis circulating tumor cells rare cell enrichment tumor heterogeneity |