| 反相HPLC双检测器法同时测定米根霉乳酸发酵液中的有机酸与葡萄糖 |
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| 引用本文： | 白冬梅,赵学明,胡宗定. 反相HPLC双检测器法同时测定米根霉乳酸发酵液中的有机酸与葡萄糖[J]. 食品与发酵工业, 2001, 27(1): 13-17 |
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| 作者姓名： | 白冬梅 赵学明 胡宗定 |
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| 作者单位： | 天津大学生物化工系, |
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| 基金项目： | 国家自然科学基金资助项目(29776035) |
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| 摘 要： | 提出了用高效液相色谱法同时测定米根霉乳酸发酵液中的有机酸和葡萄糖。用Wakosil Ⅱ 5C18RS(4 6mm× 150mm ,5μm)色谱柱 ,以 0 0 1mol/L磷酸水溶液 (pH2 5)为流动相 ,流速 1mL/min ,利用有机酸及葡萄糖对紫外光吸收强弱的差异 ,采用双检测器系统 ,以示差折光检测器检测葡萄糖 ,以可变波长检测器检测有机酸 ,一次进样可同时定性及定量分析待测样中的有机酸及葡萄糖 ,每一样品的分析过程不超过 10min ,对于分析数量较多的样品 ,可及时了解乳酸发酵过程的变化 ,方法的优越性尤为突出
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| 关 键 词： | 反相高效液相色谱 双检测器 米根霉 乳酸发酵液 有机酸 葡萄糖 |
| 修稿时间： | 2000-05-13 |
Simultaneous Determination of Organic Acid and Glucose in Lactic Acid Fermented Broth of Rhizopus oryzae by Reversed-phase High Performance Liquid Chromatography (RP-HPLC) with Double Detector |
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| Bai Dongmei Zhao Xueming Hu Zongding. Simultaneous Determination of Organic Acid and Glucose in Lactic Acid Fermented Broth of Rhizopus oryzae by Reversed-phase High Performance Liquid Chromatography (RP-HPLC) with Double Detector[J]. Food and Fermentation Industries, 2001, 27(1): 13-17 |
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| Authors: | Bai Dongmei Zhao Xueming Hu Zongding |
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| Abstract: | A RP HPLC method for simultaneous determinition of organic acid and glucose in lactic acid fermented broth of Rhizopus oryzae was developed. Wakosil Ⅱ 5C18RS column was used and mobile phase was 0.01?mol/L phosphoric acid (pH?2.5) with flow rate of 1.02mL/min. Variable Wavelength Detector (VWD) and Refractive Index Detector (RID) would determine organic acid and glucose in samples within 10 min, respectively. This method was proved a rapid, effective way to determinate the organic acid and glucose in lactic acid fermented broth of Rhizopus oryzae. |
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| Keywords: | reversed phase high performance liquid chromatography double detector Rhizopus oryzae lactic acid fermented broth organic acid glucose |
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