Changes in lipid composition of germinating barley embryo |
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Authors: | Gunhild Holmer Robert L Ory Carl-Erik Høy |
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Affiliation: | (1) Department of Biochemistry and Nutrition, Polytechnic Institute of Denmark, 2800 Lyngby, Denmark;(2) Present address: Southern Regional Research Laboratory, P.O. Box 19687, 70119 New Orleans, La |
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Abstract: | Barley seeds,Hordeum vulgare, var. Kenia, were dissected before and after 5 days of germination, to distinguish between the scutellum, the coleoptile
half of the embryo and the coleorhiza half of the embryo. Total lipids were extracted from each fraction and analyzed by thin
layer chromatography and gas liquid chromatography. In tissues from the coleoptile and coleorhiza halves of the embryo there
was a concurrent disappearance of triglycerides with a marked increase of esterified sterols and esterified sterol glucosides.
In the scutellum there was also a change in triglycerides, but the variations in contents of esterified sterols and esterified
sterol glucosides were much smaller. Mono- and digalactolipids were virtually absent from embryonic tissue. The amounts of
linoleic and linolenic acids in esterified sterol glucosides were increased after 5 days of germination in all the embryonic
tissues, especially in the coleoptile half. In sterol esters, linoleic acid comprised nearly half of the total fatty acids,
and the desaturation after 5 days of germination was much less pronounced. |
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