利用响应面法优化L-苏氨酸发酵条件

采用响应面设计方法对E.coli TRFC苏氨酸发酵培养基及培养条件进行了优化。用部分因子分析法研究了原始发酵培养基及培养条件对响应值的影响程度,发现蔗糖的质量浓度及接种量对苏氧酸产量的影响显著。利用最陡爬坡实验、中心旋转组合设计结合响应面分析确定了蔗糖的质量浓度及接种量(58.739 g/L,3.46%)。在优化条件下进行5L发酵罐实验,L-苏氨酸的产量达到121.20 g/L,比未优化条件下提高了12.43%。

Optimization of L-threonine Acid Production by Response Surface Methodology

Optimal medium composition and culture conditions for L-threonine production via E.coli TR- FC fermentation was studied by Response Surface Methodology (RSM).The impact of initial medium con- stituents and conditions on L-threonine production were investigated by Fractional Factorial Design(FFD), discovering that both sucrose and inoculum volume have a substantial influence on L-threonine production. The optimal concentrations of sucrose and inoculum volume was defined as 58.739 g/L and 3.46% by the steepest ascent design,Central Composite Rotatable Design (CCD) and Response Surface Analysis.The L- threonine yield was promoted by 12.43% from 107.80 g/L to 121.20 g/L in a 5L fermentor under optimized conditions.