| G8r菌产人参皂苷糖苷酶的酶性质及酶水解作用 |
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| 引用本文： | 陈娇娇,孙键,金凤燮,鱼红闪. G8r菌产人参皂苷糖苷酶的酶性质及酶水解作用[J]. 大连工业大学学报, 2011, 30(1): 13-17 |
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| 作者姓名： | 陈娇娇 孙键 金凤燮 鱼红闪 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁大连,116034 |
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| 基金项目： | 国家自然科学基金，辽宁省科学技术基金，辽宁省教育厅创新团队项目 |
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| 摘 要： | 对由Absidia sp.G8r菌产的人参皂苷糖苷酶(Glc8)进行了分离纯化,并对其酶性质和酶水解作用进行了研究.结果表明,该酶的分子质量约为72 ku,酶反应的最适温度和pH分别为30 ℃和5.0,在20～60 ℃、pH 3.0～7.0条件下稳定性较好.该酶水解人参皂苷Rb1生成人参皂苷C-K,水解过程是分步进行的...
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| 关 键 词： | 人参皂苷 人参皂苷糖苷酶 酶性质 水解作用 |
Characterization of ginsenoside-glyosidase from Absidia sp.G8r |
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| CHEN Jiao-jiao,SUN jian,JIN Feng-xie,YU Hong-shan. Characterization of ginsenoside-glyosidase from Absidia sp.G8r[J]. Journal of Dalian Dalian Polytechnic University, 2011, 30(1): 13-17 |
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| Authors: | CHEN Jiao-jiao SUN jian JIN Feng-xie YU Hong-shan |
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| Affiliation: | School of Bioengineering,Dalian Polytechnic University,Dalian 116034,China |
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| Abstract: | The ginsenoside-glyosidase produced by Absidia sp.G8r was purified and characterized.The result indicated that its molecular weight is about 72 ku and the optimal temperature and pH for enzyme reaction were 30 and 5.0 respectively Enzyme is stable at 20-60 ℃,pH 3.0-7.0.Ions K+,Na+,Ca2+,Mg2+,Zn2+ had no effect on the enzyme activity,while Cu2+ inhibited the enzyme activity.The enzyme hydrolyze Rb1 to C-K,in which Rb1 was hydrolyzed to Rd first in about 8 h,then Rd was hydrolyzed to F2 in about 18 h,and after that F2 was hydrolyzed to C-K.The Km of the enzyme are 5.488,5.176,5.495 mmol/L,the Vmax of the enzyme are 1.524,0.904,0.447 mmol/(L·h). |
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| Keywords: | ginsenoside ginsenoside-glyosidase enzymatic characteristics hydrolysis |
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