生物活性肽—酪蛋白磷酸肽（CPP）的研制

摸索了2709碱性蛋白酶水解酪蛋白制备CPP的最佳工艺条件：底物浓度10%、用酶量1500u/g反应温度45℃、pH10.5、反应时间150min。采用选择沉淀法分离CPP，在pH4.5添加1.1?Cl2(w/v)和50%（v/v)乙醇，室温沉淀4h ,得到CPP产品的N/P、得率分别为6.39%和13.94%。

Optimal Parmeters Study on Processing Casein Phosphopeptide

In this study, alcalase was used for producing CPP by hydrolyzing casein. The optimum conditions: casein concentration 10％, enzyme usage 1500u/g substrate, temperature 45℃, pH10. 5 and time 150mins were determined. A selective precipitation procedure was also used to isolate CPP from the hydrolyzate of casein. The N/P ratio and the yield of CPP obtained by adding 1.1％ CaCl2 (w / v ) and 50％ (v / v ) alcohol to the hyrolyzate at pH4. 5 were 6. 39％ and 13.94％ respectively.