| 基于同步扫描相机的荧光寿命测量系统研究 |
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| 引用本文： | 邵永红,李恒,王岩,屈军乐,牛憨笨. 基于同步扫描相机的荧光寿命测量系统研究[J]. 深圳大学学报(理工版), 2009, 26(4) |
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| 作者姓名： | 邵永红 李恒 王岩 屈军乐 牛憨笨 |
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| 作者单位： | 1. 深圳大学光电工程学院,光电子器件与系统教育部重点实验室,广东省光电子器件与系统重点实验室,深圳,518060
2. 深圳大学光电工程学院,光电子器件与系统教育部重点实验室,广东省光电子器件与系统重点实验室,深圳,518060;西安工业大学光电工程学院,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目，广东省科技计划资助项目 |
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| 摘 要： | 建立一台基于同步扫描相机的双光子激发荧光寿命显微测量系统,同步扫描相机的重复工作频率为76 MHz,利用钛宝石飞秒激光器作为光源,通过可调延时器和标准具对扫描相机的时间分辨率、扫描速度以及非线性等进行标定.该系统的时间分辨率为9 ps,非线性小于4%,量程为2.8 ns.测量了荧光染料Rose Bengal(RsB)的荧光衰减曲线,通过最小二乘法对荧光的衰减曲线进行拟合,得到RsB的荧光寿命为763 ps,与标准荧光染料对比一致.
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| 关 键 词： | 荧光显微技术 荧光寿命 双光子激发 同步扫描相机 扫描速度 时间分辨率 |
A fluorescence lifetime spectrometer based on a synchroscan streak camera |
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| SHAO Yong-hong,LI Heng,WANG Yan,QU Jun-le,Hanben NIU. A fluorescence lifetime spectrometer based on a synchroscan streak camera[J]. Journal of Shenzhen University(Science &engineering), 2009, 26(4) |
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| Authors: | SHAO Yong-hong LI Heng WANG Yan QU Jun-le Hanben NIU |
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| Abstract: | A two-photon excitation fluorescence lifetime spectrometer based on a synchroscan streak camera was presented. The spectrometer was calibrated using a Titanium: Sapphire femtosecond laser in conjunction with a custom-made synchronized streak circuit, tunable delay generator and a set of etalons. Experimental results show that the temporal resolution of this spectrometer is 9 ps, nonlinearity is less than 4% , and the sweep range is 2. 8 ns. Fluorescence decay profile of fluorescent dye Rose Bengal ( RsB) is recorded with the spectrometer. Nonlinear least square fitting of the decay profile gives that the lifetime of RsB is 763 ps, consistent with standard fluorescence dyes. |
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| Keywords: | fluorescence microscopy fluorescence lifetime two-photon excitation synchroscan streak camera sweep speed temporal resolution |
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