| 异养培养光合细菌产5-氨基乙酰丙酸发酵条件的研究 |
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| 引用本文： | 赵春晖,穆江华,林建平,岑沛霖. 异养培养光合细菌产5-氨基乙酰丙酸发酵条件的研究[J]. 精细化工, 2003, 20(11): 648-651 |
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| 作者姓名： | 赵春晖 穆江华 林建平 岑沛霖 |
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| 作者单位： | 浙江大学,化学工程与生物工程学系,浙江,杭州,310027 |
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| 摘 要： | 从废水处理厂的活性污泥中筛选出产5 氨基乙酰丙酸(ALA)的红假单胞菌原始菌株,可产生7 35mg/L的ALA,采用亚硝基胍(NTG)以及紫外线对该菌株进行诱变处理,得到产量为19 12mg/L的菌株C-1。在摇瓶实验过程中,对培养基组成和培养条件进行了初步优化,使菌株产ALA的能力达到了38 9mg/L。
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| 关 键 词： | 5氨基乙酰丙酸 红假单胞菌 诱变育种 培养条件 |
| 文章编号： | 1003-5214(2003)11-0648-04 |
Study of the Shake Bottle Fermentation for 5-Aminolevulinic Acid Production by Rhodopseudomonas |
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| ZHAO Chun-hui,MU Jiang-hua,LIN Jian-ping,CEN Pei-lin. Study of the Shake Bottle Fermentation for 5-Aminolevulinic Acid Production by Rhodopseudomonas[J]. Fine Chemicals, 2003, 20(11): 648-651 |
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| Authors: | ZHAO Chun-hui MU Jiang-hua LIN Jian-ping CEN Pei-lin |
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| Abstract: | Rhodopseudomonas was screened from active sludge in a local wastewater treatment facility.The productivity of 5-aminolevulinic acid (ALA) is 7.35 mg/L under aerobic and dark cultivation.After mutation by N-methyl-N′-nitro-nitrosoguanidine(NTG) and ultraviolet light,a mutant,C-1,was obtained with ALA productivity of 19.12 mg/L.After preliminary optimization of medium composition and cultivation conditions,the ALA productivity increased to 38.9 mg/L. |
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| Keywords: | 5-aminolevulinic acid Rhodopseudomonas mutation cultivation condition |
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