High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates |
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| Authors: | Hasegawa Toshimi Hayashi Manabu Nomura Kazuki Hayashi Mayumi Kido Miyuki Ohmori Tsuneo Fukuda Masao Iguchi Akinori Ueno Shigeaki Shigematsu Toru Hirayama Masao Fujii Tomoyuki |
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| Affiliation: | Department of Food Science, Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1 Higashijima, Akiha-ku, Niigata, Niigata 956-8603, Japan. |
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| Abstract: | Using microplates as pressure and cultivation vessels, a high-throughput method was developed for analyzing the high-pressure inactivation kinetics of microorganisms. The loss of viability from a high-pressure treatment, measured based on the growth delay during microplate cultivation, showed reproducibility with the conventional agar plate method and was applicable for the kinetics analysis. |
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| Keywords: | Microplate High-throughput High-pressure Microbial inactivation Escherichia coli Saccharomyces cerevisiae |
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