| 电化学发光免疫传感器检测相思子毒素研究 |
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| 引用本文： | 刘冰,童朝阳,郝兰群,穆晞惠,刘威,黄启斌. 电化学发光免疫传感器检测相思子毒素研究[J]. 传感器与微系统, 2013, 32(5) |
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| 作者姓名： | 刘冰 童朝阳 郝兰群 穆晞惠 刘威 黄启斌 |
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| 作者单位： | 防化研究院国民核生化灾害防护国家重点实验室,北京,102205 |
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| 摘 要： | 以磁性微球固定相思子毒素多抗制备捕获探针,以三联吡啶钌标记相思子毒素单抗作为发光探针,两者与相思子毒素发生特异性免疫反应形成夹心复合物,成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素,浓度在0.1～1000μg/L范围内与电化学发光强度呈良好的对数线性关系,拟合方程为lg Y=0.763 lg X+0.562(R=0.9903,N=7,P<0.0001),检出限为0.1μg/L。
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| 关 键 词： | 电化学发光 免疫传感器 检测 相思子毒素 |
Research on abrin detection by electrochemiluminescence immunosensor |
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| LIU Bing , TONG Zhao-yang , HAO Lan-qun , MU Xi-hui , LIU Wei , HUANG Qi-bin. Research on abrin detection by electrochemiluminescence immunosensor[J]. Transducer and Microsystem Technology, 2013, 32(5) |
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| Authors: | LIU Bing TONG Zhao-yang HAO Lan-qun MU Xi-hui LIU Wei HUANG Qi-bin |
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| Abstract: | Polyclonal antibody(PcAb)of abrin is immobilized on magnetic microbeads as capture probe.An efficient approach for abrin detection by electrochemiluminescence(ECL)immunosensor is developed based on specific immunological reaction between immobilized PcAb,abrin and Ru(bpy)2+3-labeled monoclonal antibody(McAb),which forms sandwich immunocomplex.Then the target abrin is determined by ECL.A good logarithmic linear relationship between ECL intensity and the concentration of abrin at a range of 0.1 ~1 000 μg/L is obtained,fitting equation is lg Y=0.763 lg X+0.562(R=0.990 3,N=7,P<0.000 1)and the detection limit is 0.1 μg/L. |
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| Keywords: | electrochemiluminescence(ECL) immunosensor detection abrin |
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