Increased sedimentation of a Pseudomonas–Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates |
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作者姓名: | Chang Liu Lin Qi Songyuan Yang Yun He Xiaoqiang Jia |
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作者单位: | 1.Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;2.Key Laboratory of Systems Bioengineering(Tianjin University), Ministry of Education, Tianjin 300072, China;3.SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China |
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基金项目: | the National Basic Research Program of China;the National Natural Science Foundation of China;Tianjin Research Program of Application Foundation and Advanced Technology;Tianjin Key Research & Development Program |
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摘 要: | Concerns about feasibility,separability,settleability,efficiency once hampered studies on polyhydroxyalkanoates(PHAs) production,which mainly focused on single strain microorganism or activated sludge rather than artificial microbial consortia.Here,a medium chain length PHAs(mcl-PHAs) producing Pseudomonas-Saccharomyces consortium with xylose as the main substrate was studied.Mcl-PHAs accumulation increased from 12.69 mg·L~(-1) to 152.3 mg·L~(-1) without any optimization method.The presence of Saccharomyces cerevisiae,though in a relatively low concentration,improved the sedimentation of cell mass of the mixed culture by 60%.Reasons for better sedimentation of the consortium were complex:first,the length of Pseudomonas putida increased two to three times in the consortium;second,the positive surface charge of P.putida was neutralized by S.cerevisiae;third,the adhesion proteins on the surface of S.cerevisiae interacted with the P.putida.
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关 键 词: | Medium chain length polyhydroxyalkanoates Pseudomonas-Saccharomyces consortium Sedimentation Xylose |
收稿时间: | 3 August 2018 |
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