| Extraction and purification of magnetic nanoparticles from strain of Leptospirillum ferriphilum |
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| 作者单位： | School of Resources Processing and Bioengineering Central South University，2.School of Life Science，Hunan University of Science and Technology，Xiangtan 410201，China，School of Resources Processing and Bioengineering，Central South University，School of Resources Processing and Bioengineering，Central South University，School of Resources Processing and Bioengineering，Central South University，2.School of Life Science，Hunan University of Science and Technology，Xiangtan 410201，China，School of Resources Processing and Bioengineering，Central South University，School of Resources Processing and Bioengineering，Central South University，Changsha 410083，China，Changsha 410083，China，Changsha 410083，China，Changsha 410083，China，Changsha 410083，China，Changsha 410083，China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | 1Introduction From1975,there is much interest in finding magne-totactic bacteria1],because bacterial magnetosome particles,unlike those produced chemically,have a consistent shape,a unique crystallography,a narrow size distribution within the single magn…
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Extraction and purification of magnetic nanoparticles from strain of Leptospirillum ferriphilum |
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| Authors: | GAO Jian XIE Jian-ping DING Jian-nan KANG Jian CHENG Hai-na QIU Guan-zhou |
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| Abstract: | The magnetic nanoparticles were extracted from Leptospirillum ferriphilum,strain YSK,isolated from acid mine drainages by treatment with sodium dodecyl sulfate(SDS)and centrifugation through a sucrose density gradient.Transmission electron microscopy(TEM)indicates that the nanoparticles are approximately spherical with a mean diameter of 44 nm,and magnetite crystals in this size range are single magnetic domains.Energy-dispersive X-ray analysis shows that the nanoparticles primarily contain two kinds of elements,iron and oxygen.Thus it can be concluded that the magnetic particles are magnetosomes.Generally,it is thought that cellular magnetotaxis is a direct consequence of the cell possessing magnetosomes.The discovery of magnetosomes in strain YSK can provide the theoretical basis for screening efficient bioleaching bacteria which are specific to different magnetic minerals under an outer magnetic field. |
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| Keywords: | Leptospirillumferriphilum strain YSK SDS sucrose density gradient magnetosomes magnetotaxis |
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