Automation of environmental ELISAs |
| |
Authors: | C B Shumate J E Johnson D A Fitzpatrick C Charan |
| |
Affiliation: | 1. Hamilton Company, 4970 Energy Way, Reno, NV, 89502, USA.;2. Quantix Systems, 2611 Branch Pike, Cinnaminson, NJ, 08077, USA.;3. Midwest Research Institute, 625-B Clyde Avenue, Mountain View, CA, 94043, USA, |
| |
Abstract: | ELISAs for pesticides and herbicides in environmental and
agricultural samples are becoming very important in screening
applications 1-3]. Traditional chromatographic methods are
expensive and results need long turnaround times, making them
incompatible with rapid on-site decision making. ELISA methods
have been shown to meet or exceed the performance of gas
chromatography—they offer rapid low-cost analysis, thereby
increasing the frequency of sampling and enhancing data quality.
Automated ELISA workstations allow the full benefit of these kits
to be realized. Sample preparation, reagent pipetting, incubation,
and photometric evaluation can be performed without user
intervention. Reliability is increased through the elimination of
operator error, better accuracy and precision, and often higher speed.
Much larger batch sizes are possible and these systems can provide
sample tracking with report generation for documentation
requirements. In this paper the manual procedures and ELISA
methods are compared and some critical aspects of automating these
ELISA kits are discussed. |
| |
Keywords: | |
|
|