反相高效液相色谱法测定去屑香波中去屑止痒剂Octopirox的含量

采用反相高效液相色谱法测定了香波中去屑止痒剂Octopirox。在Symmetry C18(5um,4.6mmi.d.X250mm)色谱柱上，以乙腈：水（含0.02mol磷酸和0.5mmolEDTA二纳）]=70：30的溶液为流动相，流速为0.8mL/min，紫外检测波长为305nm，柱温为35℃下检测，样品用流动相搅拌、超声提取，回收率为97.2%-98.3%，样品测试相对偏差为0.1%-0.4%。具有操作简便，分析速度快和重复性好等特点。

Determination of Anti Dandruff Agent Octopirox in Shampoo by Reversed Phase Liquid Chromatography

A method based on reversed phase liquid chromatography is developed for the determination of Octopirox, an antidandruff agent in shampoo. A symmetry C18 column (5 μm, 4.6 mm i. d. X250 mm) was used at temperature of 35 ℃, mobile phase with flow rate of 0.8 mL/min was φ acetonitrile: water ( containing0.02 mol orthophpnoshoric acid and 0.5 mmolEDTS disodium salt) ] ＝ 70:30 and UV detection at 305 nm. Samples were extracted with mobile phase by stirring and ultrasonic method. The average recoveries of Octopirox were 97.2 % ～ 98.3 %. The relative standard deviations were 0. 1% ～ 0.4 %. The method is simple, rapid and reproducible.