胶质瘤细胞系摄取BPA的实验研究

目的:探讨BPA(2,2-双(4-羟基苯基)丙烷,Bisphenol A)浓度和温度对胶质瘤细胞系摄取和析出10B的影响.方法:将C6和U251两种胶质瘤细胞系,及大鼠正常脑胶质细胞培养在含不同浓度BPA(10B浓度分别为20、40、60、80、100 μg/mL)的培养基中24h后,采用感应耦合等离子体原子发射光谱(ICP-AES)法测定细胞内硼的含量;将C6细胞培养在含不同浓度BPA的培养基中培养24h后,更换为不含10B培养基,在不同温度条件(4、25、37℃)下继续培养,并分别于换液后的1、2、3h,用ICP-AES方法检测细胞内的硼含量.结果:细胞内硼浓度随培养基中BPA浓度的增加而增高,胶质瘤细胞内10B浓度约为正常胶质细胞的2.2倍;温度越高,细胞内硼析出速度越快.结论:BPA对胶质瘤细胞系具有一定亲和力;细胞对10B的析出速率具有温度依赖性.

Experimental study on BPA uptake of glioma cell lines

Objective: to evaluate the incorporation of BPA by glioma cell lines,and to observe its relationship with the temperature and the concentration of BPA.Methods: C6,U251 and rat astrocyte cells were incubated in a culture medium,in which 10B concentration was 20,40,60,80,100 μg/mL for 24 h.Boron concentration in the cells was measured induced couple plasma-atomic emission spectroscopy(ICP-AES).C6 cells were pre-incubated for 24 h with different boron concentrations in growth medium.Then the mediums were changed to boron-free ones,and boron content was assessed after 1,2,3 h.Results: The content of the 10B in cells was increasing with the increasing concentration of BPA,and the boron concentration ratios of glioma cells to astrocyte are 2.2.The BPA efflux is slower at the lower temperature.Conclusion: BPA has a selectivity for glioma cells,and the results of the efflux assay confirm the temperature dependence of the BPA transport out of the cells.