| 猪皮提取胶原的研究 |
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| 引用本文： | 金勇,徐社阳,刘宗惠,魏德卿.猪皮提取胶原的研究[J].精细化工,2001,18(5):302-304. |
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| 作者姓名： | 金勇 徐社阳 刘宗惠 魏德卿 |
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| 作者单位： | 中国科学院成都有机化学研究所, |
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| 基金项目： | 中国科学院 1999年度“西部之光”基金&& |
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| 摘 要： | 对以新鲜猪皮为原料 ,采用氯化钠 /三羟甲基氨基甲烷 -盐酸缓冲液 (pH =7.5 )为萃取液以不同浓度 (0 45mol/L及 1 0 0mol/L)分两次萃取 ,用冰醋酸 /氯化钠沉淀 ,然后用冰醋酸溶解精制纯化后再中和提取胶原的新方法进行了研究。所提取胶原的纯度以羟脯氨酸质量分数为基准 ,采用分光光度法测定为 95 7%。其结构为红外光谱所确证。
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| 关 键 词： | 猪皮 提取 胶原 |
| 文章编号： | 1003-5214(2001)05-0302-03 |
Extraction of Collagen from Pig Skin |
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| JIN Yong,XU She-yang,LIU Zong-hui,WEI De-qing.Extraction of Collagen from Pig Skin[J].Fine Chemicals,2001,18(5):302-304. |
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| Authors: | JIN Yong XU She-yang LIU Zong-hui WEI De-qing |
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| Abstract: | In this paper,a new technology for extraction of collagen from pig skin was studied.NaCl/Tris-HCl buffer(pH=7.5) was used as extractant to extract collagen two times,firstly using 0.45 mol/L and secondly 1.00 mol/L NaCl/Tris-HCl buffer.Then NaCl/HAc solution was used as precipitant and HAc as solvent to refine collagen.The purity of collagen was 95.7% based on the hydroxyproline content determined by UV.The struture of collagen was identified by FTIR. |
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| Keywords: | pig skin extraction collagen |
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