| DL-苯丙氨酸的酶法拆分研究 |
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| 引用本文： | 何仕国,俞一军,许文松.DL-苯丙氨酸的酶法拆分研究[J].化学反应工程与工艺,2004,20(1):64-69. |
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| 作者姓名： | 何仕国 俞一军 许文松 |
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| 作者单位： | 浙江大学化学工程与生物工程系,浙江,杭州,310027;浙江大学化学工程与生物工程系,浙江,杭州,310027;浙江大学化学工程与生物工程系,浙江,杭州,310027 |
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| 摘 要： | 利用氨基酰化酶能立体专一地水解氨基酰化物的特点,选择性地水解N-乙酰-L-苯丙氨酸得到L-苯丙氨酸,经分离后再水解N-乙酰-D-苯丙氨酸得到D-苯丙氨酸。研究中分别考察了pH值、温度、酶用量、底物浓度和钴离子对反应的影响。在优选条件下(底物浓度为0.1mol·L-1,pH7.0,温度37℃,酶用量为w(酶)/w(底物)=1∶41.4,加入浓度为1×10-3mol/L的Co2+,将N-乙酰-D-苯丙氨酸用有机溶剂结晶和盐酸水解10h)得到光学纯度OP=98.8%的L-苯丙氨酸,收率70.3%;OP=96%的D-苯丙氨酸,收率63%。
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| 关 键 词： | 苯丙氨酸 氨基酰化酶 拆分 |
| 文章编号： | 1001-7631(2004)01-0064-06 |
| 修稿时间： | 2003年3月23日 |
Study on the Resolution of DL-Phenylalanine by Aminoacylase |
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| He Shiguo Yu Yijun Xu Wensong.Study on the Resolution of DL-Phenylalanine by Aminoacylase[J].Chemical Reaction Engineering and Technology,2004,20(1):64-69. |
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| Authors: | He Shiguo Yu Yijun Xu Wensong |
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| Abstract: | The L-phenylalanine and D- phenylalanine has been be prepared in high optical purity by treating N-acetylphenylalanine in water using free aminoacylase as a catalyst. A number of influencing factors to the resolution reaction were discussed, including pH value, reaction temperature, amount of aminoacylase, concentration of substrate and Co~(2+). In end, L-phenylalanine was obtained with 98.8% optical purity in 70.3% yield, D- phenylalanine was obtained with 96% optical purity in 63% yield. |
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| Keywords: | phenylalanine resolution aminoacylase |
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