| 热带假丝酵母肉毒碱乙酰转移酶酶活测定及重组菌的性质 |
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| 引用本文： | 张剑,高弘,李春,曹竹安. 热带假丝酵母肉毒碱乙酰转移酶酶活测定及重组菌的性质[J]. 化工学报, 2005, 56(3): 511-517 |
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| 作者姓名： | 张剑 高弘 李春 曹竹安 |
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| 作者单位： | 清华大学化学工程系生物化工研究所,北京,100084;清华大学化学工程系生物化工研究所,北京,100084;清华大学化学工程系生物化工研究所,北京,100084;清华大学化学工程系生物化工研究所,北京,100084 |
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| 基金项目： | 国家重点基础研究发展规划项目(2003CB716007)，国家自然科学基金重点项目 (20036010).~~ |
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| 摘 要： | 建立了快速部分提纯和测定热带假丝酵母中肉毒碱乙酰转移酶(carnitine acetyltransferase，CAT)酶活的方法.并考察了重组热带假丝酵母的CAT酶活、产酸能力及烷烃转化率.结果表明，重组菌的CAT酶活比原始菌降低了45%，胞内二元酸通过β-氧化的无益消耗得到有效抑制.相应地，重组菌的产酸能力和烷烃转化率分别提高了11%和12%.
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| 关 键 词： | 肉毒碱乙酰转移酶 β-氧化 长链二元酸 重组菌 热带假丝酵母 |
| 文章编号： | 0438-1157(2005)03-0511-07 |
| 收稿时间： | 2003-12-29 |
| 修稿时间： | 2004-4-5 |
Acetyltransferase activity in Candida tropicalis and characteristics of CAT disruption recombinant |
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| ZHANG Jian,GAO Hong,LI Chun,CAO Zhu'an. Acetyltransferase activity in Candida tropicalis and characteristics of CAT disruption recombinant[J]. Journal of Chemical Industry and Engineering(China), 2005, 56(3): 511-517 |
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| Authors: | ZHANG Jian GAO Hong LI Chun CAO Zhu'an |
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| Abstract: | A rapid method of partially purifying carnitine acetyltransferase (CAT) from Candida tropicalis and determination of its enzyme activity was established.The characteristics of CAT-disruption recombinant of Candida tropicalis were also studied.It was confirmed that the specific activity of CAT of this recombinant was approximately half of that of its corresponding wild type. Therefore, the β-oxidation pathway of dicarboxylic acid was effectively suppressed. Correspondingly, the dicarboxylic acid productivity of this recombinant was increased by 11% and its alkane conversion rate was also increased by 12% in average. |
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