中性和碱性蛋白酶协同酶解大豆分离蛋白研究

采用中性和碱性蛋白酶协同酶解大豆分离蛋白制备大豆多肽,采用茚三酮分析法测定酶解液中氨基氮含量以判断其酶解效率。影响大豆分离蛋白酶解主要因素有中性与碱性蛋白酶用量比、酶解pH值、酶解温度、酶解时间,通过单因素和优化酶解条件正交试验分析,筛选出酶解最适实验条件:中性蛋白酶与碱性蛋白酶用量比为1∶3、温度55℃、pH 8.5、酶解时间6 h;在此条件下酶解,氨基氮含量为15.86 mg/g。

Study on soybean protein isolate is collaborative hydrolysis by neutral and alkali protease

That soybean protein isolated is collaborative hydrolyzed by neutral and alkali protease can produce soybean peptide,To judge its hydrolysis efficiency,amino nitrogen content in the enzyme solution is determined with triketohydrindene hydrate colorimetric method.The main factors such as neutral and alkali protease quantity ratio,pH,the temperature of reaction,the time of reaction are discussed in emphasis.The optimal conditions of hydrolysis were determined by single factor and orthogonal experiments.The optimized conditions of hydrolysis are neutral and alkali protease ratio for 1∶3,55 ℃,pH 8.5.After 6 hours of hydrolysis,amino nitrogen content was 15.86 mg/g.