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Biological synthesis of very small silver nanoparticles by culture supernatant of Klebsiella pneumonia: The effects of visible-light irradiation and the liquid mixing process
Authors:Narges Mokhtari  Seyedali Seyedbagheri  Khosro Abdi  Sara Minaian  Ahmad Reza Shahverdi
Affiliation:a Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
b Hydrometallurgy Research Unit, Research & Development Center, National Iranian Copper Industries Company, Sarcheshmeh, Rafsanjan, Iran
c Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
d Department of Material Science, Faculty of Engineering, Tarbiat Modares University, Tehran, Iran
Abstract:This study has investigated different visible-light irradiation's effect on the formation of silver nanoparticles from silver nitrate using the culture supernatant of Klebsiella pneumonia. Our study shows that visible-light emission can significantly prompt the synthesis of silver nanoparticles. Also, the study experimentally investigated the liquid mixing process effect on silver nanoparticle synthesis by visible-light irradiation. This study successfully synthesized uniformly dispersed silver nanoparticles with a uniform size and shape in the range of 1-6 nm with an average size of 3 nm. Furthermore, the study investigated the mechanism of the reduction of silver ions by culture supernatant of K. pneumonia, and used X-ray diffraction to characterize silver chloride as an intermediate compound. Silver chloride was prepared synthetically and used as a substrate for the synthesis of silver nanoparticles by culture supernatant of K. pneumonia. The silver nanoparticles have been prepared from silver chloride during this investigation for the first time.
Keywords:A  Nanostructures  A  Elements  A  Halides  C  Electron microscopy  C  X-ray diffraction
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