转基因番茄DNA检测芯片的研究

自19世纪70年代世界上第一例转基因植物问世以来，它对人类健康及环境的安全性问题，受到了全世界广泛的关注：本研究根据转基因番茄（Zeneea）中所转入的外源基因，选择CaMV35S启动子、NOS终止子、PG／NOS基因和内源PG基因设计特异性引物，采用多重PCR法对待测样品进行扩增，通过缺口平移法合成DIG—dUTP标记杂交探针，并制备基因芯片。在对PCR反应和扩增产物与芯片杂交条件进行优化的同时，比较了芯片检测的特异性和重复性，并对检测的灵敏度进行测试。结果表明，该方法具有较好的特异性和重复性，检测灵敏度可达0．5％，由于采用了多重PCR技术一次可同时检测多个基因，提高了检测的；隹确性和效率。

THE RESEARCH OF GENETICALLY MODIFIED TOMATO DETECTION WITH GENE CHIP

Since 1970s, the world paid great attention to the security of Genetically Modified Organism. According to the plasmid map of Genetically Modified Tomato (Zenaca), we select three exogenous gene(CaMV35S promoter, NOS terminator, PG/NOS gene) and endogenous gene (PG) as target genes, design and synthesize their primers. The probe was synthesized using the method of nick translation and labeled with DIG-dUTP. Multiplex PCR was used to amplify the target sequence in tomato sample DNA, then we hybridized the DNA chips with PCR product, at last the chips displayed the hybridization result. The DNA chip for identifying Genetically Modified Tomato was highly specific and repeatable and its sensitivity was 0.5%,meanwhile the detection efficiency was highly improved with the use of multiplex PCR. So we could detect and identify Genetically Modified Tomato rapidly and correctly.