| PCR检测沙门氏菌invA基因的灵敏度 |
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| 引用本文： | 江树勋,吴圣静,李寿崧,饶静静,江云. PCR检测沙门氏菌invA基因的灵敏度[J]. 食品科技, 2006, 0(10): 251-253 |
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| 作者姓名： | 江树勋 吴圣静 李寿崧 饶静静 江云 |
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| 作者单位： | 福建出入境检验检疫局检验检疫技术中心,福州,350001 |
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| 摘 要： | 沙门氏菌是一种重要的肠道致病菌,其invA基因编码吸附和侵袭上皮细胞表面蛋白,应用PCR扩增invA基因检测致病性沙门氏菌,并测定其PCR检测灵敏度。结果显示,扩增出的284bp大小的序列为目的条带,实验可检出的最小灵敏度为11.542pg。
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| 关 键 词： | 沙门氏菌 灵敏度 |
| 文章编号： | 1005-9989(2006)10-0251-03 |
| 修稿时间： | 2006-06-11 |
Sensitivity of PCR detection for the inva gene in salmonella |
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| JIANG Shu-xun,WU Sheng-jing,LI Shou-song,RAO Jing-jing,JIANG Yun. Sensitivity of PCR detection for the inva gene in salmonella[J]. Food Science and Technology, 2006, 0(10): 251-253 |
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| Authors: | JIANG Shu-xun WU Sheng-jing LI Shou-song RAO Jing-jing JIANG Yun |
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| Abstract: | Salmonella is one of the important intestinal pathogenic bacteria,its invA Gene codes the protein which absorbs and attacks the epithelium.This paper studies the detection of the invA gene by PCR method,and its detection sensitivity.The results shows that an expected 284bp band is successfully amplified,and the lowest limit of the sensitivity is 11.542pg. |
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| Keywords: | PCR |
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