| 里氏木霉R3液体深层发酵产纤维素酶工艺优化研究 |
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| 引用本文： | 康东亮,吕世峰,阎振丽,鲁丰雨,王芬. 里氏木霉R3液体深层发酵产纤维素酶工艺优化研究[J]. 河南工业大学学报(自然科学版), 2006, 27(5): 47-51 |
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| 作者姓名： | 康东亮 吕世峰 阎振丽 鲁丰雨 王芬 |
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| 作者单位： | 1. 河南天冠企业集团,河南,南阳,473000
2. 南阳普康集团,河南,南阳,473003 |
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| 摘 要： | 研究了温度、pH值、溶氧对里氏木霉R3液体深层发酵产纤维素酶的影响,并进行了工艺优化.用50 L全自动通气机械搅拌发酵罐,接种量10%,发酵过程采取分段控制pH值、温度和溶氧的工艺,发酵120 h酶活力最高,FPA和CMC酶活力分别达到48.9 FPIU/mL和321.68IU/mL,FPA酶活比工艺优化前提高了221%.
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| 关 键 词： | 里氏木霉 纤维素酶 液体深层发酵 |
| 文章编号： | 1673-2383(2006)05-0047-04 |
| 修稿时间： | 2006-05-09 |
STUDY ON THE TECHNICS OPTIMIZATION FOR SUBMERGED FERMENTATION OF CELLULASE WITH TRICHODERMA REESEI R3 |
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| KANG Dong-liang,L Shi-feng,YAN Zhen-li,LU Feng-yu,WANG Fen. STUDY ON THE TECHNICS OPTIMIZATION FOR SUBMERGED FERMENTATION OF CELLULASE WITH TRICHODERMA REESEI R3[J]. Journal of Henan University of Technology Natural Science Edition, 2006, 27(5): 47-51 |
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| Authors: | KANG Dong-liang L Shi-feng YAN Zhen-li LU Feng-yu WANG Fen |
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| Affiliation: | KANG Dong-liang,L(U) Shi-feng,YAN Zhen-li,LU Feng-yu,WANG Fen |
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| Abstract: | The effect of temperature,pH value and soluble oxygen on the submerged fermentation of cellulase with Trichoderma reesei R3 were studied,and technics was optimized.In a 50-litre aerate automatic fermentation tank with mechanical agitation,the dosage of inoculation was 10%,the temperature,pH value and soluble oxygen were controlled step by step.Cellulase activity of filter paper and CMC reached 48.9 FPIU/mL and 321.68 IU/mL when ferment for 120h,comparing to the original technics,cellulase activity of FPA was higher by 221%. |
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| Keywords: | Trichoderma reesei cellulase submerged fermentation |
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