肉与肉制品中食源性致病微生物快速检测技术研究进展

随着生活水平的提高, 对肉制品的需求日渐增加。然而，肉品中的食源性致病菌严重威胁着人们的生命健康。针对肉制品基质复杂、致病微生物浓度低的特点，传统的食源性微生物检测方法耗时长、操作过程复杂,不能满足现代食品检测的要求, 以分子生物学、免疫分析、生物传感器、核酸适配体为基础的快速检测方法发展迅速, 已经成为食源性致病微生物检测的主要方法。本文主要从分子生物学检测法、基于免疫的检测方法、生物传感器检测法、核酸适配体检测技术等综述肉品中食源性致病微生物的检测方法, 并总结了各种检测技术的优缺点, 为开辟新的肉品中食源性致病微生物检测方法提供参考。

Research progress on rapid detection technology of foodborne pathogenic microorganisms in meat and meat products

With the changing diet of Chinese citizens and the increasing proportion of meat and meat products in daily life, meat and meat products have become an important source of foodborne illness and public health problems. In view of the complex matrix of meat and meat products and the low concentration of pathogenic microorganisms, the traditional detection methods of foodborne microorganisms are time-consuming and complicated, which cannot meet the requirements of modern food detection. The rapid detection methods based on molecular biology, immunoassay, biosensor and nucleic acid aptamer have developed rapidly and have become the main methods for the detection of foodborne pathogenic microorganisms. This paper mainly reviewed the detection methods of foodborne pathogenic microorganisms in meat and meat products from the perspectives of molecular biology, immunoassay, biosensor and nucleic acid aptamer detection technology, etc., and summarized the advantages and disadvantages of each detection technology, which provides references for opening up new detection methods of foodborne pathogenic microorganisms in meat and meat products.