Antioxidant activities of different Teucrium montanum L. Extracts |
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| Authors: | Jasna M &#;anadanovi&#;-Brunet Sonja M Djilas Gordana S &#;etkovi&#; Vesna T Tumbas Anamarija I Mandi&#; & Vladimir M &#;anadanovi&#; |
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| Affiliation: | Organic Chemistry Department, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia &Montenegro; Department of Ophthalmology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 1–3, 21000 Novi Sad, Serbia &Montenegro |
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| Abstract: | The sequential extraction of Teucrium montanum L. was realised with five solvents of different polarities (70% methanol, petroleum ether, chloroform, ethyl acetate, and n‐butanol) and HPLC method was used for identification of phenolic compounds. The total phenolic content of the extracts was determined spectrophotometrically according to the Folin–Ciocalteau procedure and range from 0 to 296 mg g?1. The antioxidant activity of extracts was tested by measuring their ability to scavenge reactive hydroxyl radical during the Fenton reaction, using electron spin resonance (ESR) spectroscopy. Moreover, the influence of these extracts on lipid peroxyl radicals obtained during lipid peroxidation of: (1) sunflower oil (37 °C, 3 h) induced by 4,4′‐azobis(4‐cyanovaleric acid) (ACVA) and (2) liposomes induced by 2,2′‐azobis(2‐amidino‐propane)dihydrochloride (AAPH) was studied. n‐Butanol extract, because of the highest content of total phenolic compounds (296 mg g?1) had the best antioxidant activity (100% at 0.16 mg mL?1 in Fenton reaction system; 90.57% at 5 mg mL?1 in system I; 100% at 5 mg mL?1 in system II). |
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| Keywords: | Antioxidant activities hydroxyl radical lipid peroxyl radicals phenols Teucrium montanum L |
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