Separation of non-denatured proteins using semi-crosslinked polyacrylamide capillary gel electrophoresis |
| |
Authors: | XU Jian-dong HU Ding-yu BAKTUR Ren GENG Li-na and DENG Yu-lin |
| |
Affiliation: | School of Life Science, Beijing Institute of Technology, Beijing 100081, China;State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;The Chinese People's Armed Police Force Academy, Langfang, Hebei 065000, China;School of Life Science, Beijing Institute of Technology, Beijing 100081, China;School of Life Science, Beijing Institute of Technology, Beijing 100081, China;School of Life Science, Beijing Institute of Technology, Beijing 100081, China |
| |
Abstract: | This work presents an approach to build a high-performance, low-viscous and replaceable separation matrix, semi-crosslinked polyacrylamide (semi-CPA) capillary gel electrophoresis. Non-denatured basic proteins, such as lysozyme, cytochrome C, ribonuclease A and trypsin were separated. The impacts of monomer and cross-linker concentrations on protein separation were studied, and the ability of dynamic capillary inner wall coating was demonstrated. The UV absorption interference by semi-CPA gel matrix was successfully overcome by a partial filling technique, which results in sensitivity 20 times higher than other protein separation method. The excellent separation ability, reproducibility and dynamic coating ability made semi-CPA an ideal separation media in both capillary electrophoresis and microfluidic chip separation scheme. |
| |
Keywords: | capillary gel electrophoresis semi-crosslinked polyacrylamide (semi-CPA) non-denatured protein separation |
本文献已被 CNKI 维普 等数据库收录! |
| 点击此处可从《北京理工大学学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《北京理工大学学报(英文版)》下载全文 |
|