N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
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Authors: | Chen Jeen-Kuan Shen Chia-Rui Yeh Chao-Hsien Fang Bing-Shiun Huang Tung-Li Liu Chao-Lin |
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Affiliation: | Environment and Biotechnology Department, Refining and Manufacturing Research Institute, CPC Corporation, Taiwan, Chia-yi, Taiwan; E-Mail: 078450@cpc.com.tw (J.-K.C.); 075345@cpc.com.tw (B.-S.F.); 076538@cpc.com.tw (T.-L.H.). |
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Abstract: | A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation. |
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Keywords: | CDFs Chitinibacter tainanensis chitin NAG fermentation |
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